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Test ID: AMPHU Amphetamines Confirmation, Random, Urine

Reporting Name

Amphetamines Confirmation, U

Useful For

Confirming drug exposure involving amphetamines such as amphetamine and methamphetamine, phentermine, methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDMA), and methylenediaoxyethylamphetamine (MDEA)

Specimen Type


Advisory Information

1. For situations where chain of custody is required, a Chain-of-Custody Kit (T282) is available. For chain-of-custody testing, order AMPHX / Amphetamines Confirmation, Chain of Custody, Random, Urine.

2. Additional drug panels and specific requests are available. Call 800-533-1710 or 507-266-5700.

3. If urine creatinine is required or adulteration of the sample is suspected, order ADULT / Adulterants Survey, Random, Urine.

Specimen Required

Supplies: Aliquot Tube, 5 mL (T465)

Collection Container/Tube: Plastic urine container

Submission Container/Tube: Plastic, 5-mL tube

Specimen Volume: 5 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative.

Additional Information:

1. No specimen substitutions.

2. STATS are not accepted for this test.

Specimen Minimum Volume

2.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 28 days
  Ambient  28 days
  Frozen  28 days

Reference Values



Cutoff concentrations:


<25 ng/mL



<25 ng/mL



<25 ng/mL



<25 ng/mL



<25 ng/mL



<25 ng/mL reported as negative

Day(s) and Time(s) Performed

Monday through Friday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information



G0480 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
AMPHU Amphetamines Confirmation, U In Process


Result ID Test Result Name Result LOINC Value
2934 Amphetamine-by LC-MS/MS 20410-7
29278 Phentermine-by LC-MS/MS 20557-5
2550 Methamphetamine-by LC-MS/MS 16235-4
29279 Pseudoephedrine/Ephedrine-by LC-MS/MS 58707-1
29280 MDA (Ecstasy metabolite)-by LC-MS/MS 20545-0
29281 MDMA (Ecstasy)-by LC-MS/MS 18358-2
21197 Amphetamines Interpretation 69050-3

Clinical Information

Amphetamines are sympathomimetic amines that stimulate the central nervous system activity and, in part, suppress the appetite. Phentermine, amphetamine, and methamphetamine are prescription drugs for weight loss. All of the other amphetamines are Class I (distribution prohibited) compounds. In addition to their medical use as anorectic drugs, they are used in the treatment of narcolepsy, attention-deficit disorder/attention-deficit hyperactivity disorder and minimal brain dysfunction.


Because of their stimulant effects, the drugs are commonly sold illicitly and abused. Physiological symptoms associated with very high amounts of ingested amphetamine or methamphetamine include elevated blood pressure, dilated pupils, hyperthermia, convulsions, and acute amphetamine psychosis.


The presence of amphetamines in urine at concentrations greater than 500 ng/mL is a strong indicator that the patient has used one of these drugs within the past 3 days.


Methamphetamine has a half-life of 9 to 24 hours and is metabolized by hepatic demethylation to amphetamine. Consequently, a sample containing methamphetamine usually also contains amphetamine. Amphetamine has a half-life of 4 to 24 hours.


Amphetamine is not metabolized to methamphetamine; absence of methamphetamine in the presence of amphetamine indicates the primary drug of abuse is amphetamine.


3,4-Methylenedioxymethamphetamine (Ecstasy, MDMA) is metabolized to 3,4-methylenedioxyamphetamine (MDA).


The detection interval in urine for amphetamine type stimulants is typically to 3 to 5 days after last ingestion.


This test will produce true-positive results for urine specimens collected from patients who are administered Adderall and Benzedrine (contain amphetamine); Desoxyn and Vicks Inhaler (contain methamphetamine); Selegeline, and famprofazone (metabolized to methamphetamine and amphetamine); and clobenzorex, fenproporex, and mefenorex, which are metabolized to amphetamine.

Clinical Reference

1. Disposition of Toxic Drugs and Chemicals in Man. Tenth edition. Edited by RC Baselt. Foster City, CA: Biomedical Publications, 2014

2. Langman LJ et al: Clinical Toxicology. In Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. Edited by N Rifai, AR Horvath, CT Wittwer. Sixth edition. St. Louis, MO. Elsevier, 2018, pp 832-887

Analytic Time

2 days

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)


If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.

Mayo Clinic Laboratories | Therapeutics Catalog Additional Information:

mml-amphetamines, mml-substancemonitoring